Characterization of hydrogenated dentin components by advanced 1H solid-state NMR experiments

Collecting information about molecular organisation on biological materials such as bone and dentin represents a major challenge in attaining better understanding of their mechanical properties. To that end, solid state Nuclear Magnetic Resonance (ssNMR) spectroscopic study is an appropriate strategy to provide atomic structural details these amorphous composite materials. However, species like water molecules hydroxyl groups are usually observed through 1H magic angle spinning (MAS) ssNMR suffers from poor resolution due strong signal overlapping, making identification difficult. This paper proposes set experiments for characterization the main components human dentin, based homo- hetero-nuclear dipolar couplings composed mostly fast 1D experiments. The assignment assisted by straightforward sample modifications: vacuum drying, deuterium exchange demineralization. These allow hydrogen edition molecules, HPO42− OH− groups, depending localization (bound organic phase, linked apatite or at interface) dynamic behaviour. toolbox has potential important chemical physical modifications biomaterials. Molecular characterisation apatitic biomaterials biophysical techniques extremely difficult complex nature. It is, however, crucial obtain if we want understand properties relation state, example hydration levels. In this article used NMR distinguish with particular attention known role biomaterial structuring.